Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Effect of miR-384-targeting LINC00491 on proliferation, migration and invasion of tongue squamous cell carcinoma cells

Yun Deng¹ , Zhiwei Luo¹, Peilin Feng², Shuai Wang³,

¹Department of Stomatology, Chengdu Fifth People's Hospital; ²Department of Stomatology, Dayi County People's Hospital, Chengdu 611300; ³Department of Stomatology, Jintang County People's Hospital, Chengdu 610400, PR China.

For correspondence:- Yun Deng Email: Dengbinnews@126.com

Accepted: 27 January 2021 Published: 28 February 2021

Citation: Deng Y, Luo Z, Feng P, Wang S, Effect of miR-384-targeting LINC00491 on proliferation, migration and invasion of tongue squamous cell carcinoma cells. Trop J Pharm Res 2021; 20(2):249-256 doi: 10.4314/tjpr.v20i2.4

© 2021 The authors.
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Abstract

Purpose: To investigate the effect of long-chain non-coding RNA LINC00491 (LncRNA LINC00491) on the proliferation, migration and invasion of tongue squamous cell carcinoma (TSCC) cells, and the underlying mechanism.

Methods: Real-time quantitative polymerase chain reaction (qRT-PCR) was applied to determine the expressions of LINC00491 and micro-RNA-384 (miR-384). Furthermore, LINC00491 and miR-384 were transfected into CAL-27 cells, while cell cycle was analyzed using flow cytometry. Cell proliferation was determined by methyl thiazolyl diphenyl-tetrazolium (MTT) assay. Cell migration and invasion were evaluated using Transwell experiments. The relationship between LINC00491 and miR-384 was confirmed using double luciferase reporting assay, while protein expression levels of P21, Ki67, E-cadherin, N-cadherin, and vimentin were assayed with Western blotting.

Results: The expression of LINC00491 increased in TSCC tissues (p < 0.05). The proportion of cells in G1-phase increased, while the proportion of cells in S-phase decreased (p < 0.05). There was decrease in cell survival, cell migration and cell invasion (p < 0.05). The protein expression levels of Ki67, N-cadherin, and vimentin were lowered, while those of P21, E-cadherin protein were increased (p < 0.05). Transfection of LINC00491 and miR- 384 reduced the proportion of cells in G1 phase, but increased the proportion of cells in S-phase (p < 0.05). Moreover, cell survival, migration and invasion were increased. The protein expressions of Ki67, N-cadherin, and vimentin rose, while those of P21 and E-cadherin decreased (p < 0.05).

Conclusion: LINC00491 promotes the proliferation, migration and invasion of TSCC cells by inhibiting miR-384. This finding provides a potential target for the treatment of TSCC.

Keywords: LINC00491, MiR-384, Tongue squamous cell carcinoma, Proliferation, Migration, Invasion